Coding

Part:BBa_K4759019

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


TF

Recombinant protein expression plays an important role in promoting the development of modern biological technology. By using the host to achieve exogenous protein expression, it provides a powerful tool for studying the structure and function of some important proteins, especially the human source proteins. In order to enable subsequent structure-function studies, the obtained recombinant proteins must be soluble, stable, properly folded as well as biologically active. However, in practical studies, these often become obstacles to obtaining an ideal recombinant protein. The solubility of the recombinant protein expression became the primary addressed problem.Trigger Factor Is a prokaryotic ribosome-binding partner protein capable of promoting co-translational folding of nascent peptide chains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 73
    Illegal SapI.rc site found at 721
    Illegal SapI.rc site found at 834
    Illegal SapI.rc site found at 1090


[edit]
Categories
Parameters
None